Intermediate

Part:BBa_K260015:Design

Designed by: Kaj Bernhardt   Group: iGEM09_BIOTEC_Dresden   (2009-10-18)

P_FRT_dhfr. (strong promoter, translated FRT site, trimethoprim resistance)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 79
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 79
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 79
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A strong promoter was necessary and we use BBa_J23100. An appropriate reading frame for the FRT site had to be found to prevent premature Stop-codons and hydrophobic amino acid residues. Further, the gene encoding TmpR was codon-optimised.


Source

This part was synthesised by "Mr Gene" (Geneart) and is available in the following plasmid backbones:

BBa_K260004: pMA-@CC2FosMB-00500bp

BBa_K260005: pMA-@CC2FosMB-01000bp

BBa_K260006: pMA-@CC2FosMB-02000bp

BBa_K260007: pMA-@CC2FosMB-05000bp

BBa_K260008: pMA-RQ-@CC2FosMB-10000bp

References